TABLE 1.
Most representative sequences of bacterial clones found in October in the three stations of the Reigous Creek (S1, COWA, and COWG) and in two stations in January (S1 and COWG)c
| Sampling period | Station | Clone | Accession no. | Phylogenetic group | Closest relative (postulated metabolism) | % Similarity | Relative abundance of clones (%)a | T-RFs (bp)b |
|---|---|---|---|---|---|---|---|---|
| October | S1 | S1Oct9 | AJ877944 | δ-Proteobacteria | Desulfobacterium indolicum (sulfate reduction) | 96 | 10 | 202 |
| S1Oct7 | AJ877952 | γ-Proteobacteria | A. ferrooxidans (sulfide/iron oxidation and iron reduction) | 99 | 8 | 251-197 | ||
| S1Oct43 | AJ877956 | Actinobacteria | Actinomycetales spp. (sulfide/iron oxidation) | 94 | 8 | 160 | ||
| S1Oct11 | AJ877924 | β-Proteobacteria | Gallionella ferruginea (iron oxidation) | 94 | 5 | 217 | ||
| COWA | COWAOct18 | AJ877926 | β-Proteobacteria | Gallionella ferruginea (iron oxidation) | 95 | 28 | 217 | |
| COWAOct77 | AJ877946 | δ-Proteobacteria | Desulfobacterium indolicum (sulfate reduction) | 94 | 8 | 202 | ||
| COWG | COWGOct61 | AJ877929 | β-Proteobacteria | Gallionella ferruginea (iron oxidation) | 94 | 22 | 217 | |
| COWGOct52 | AJ877947 | δ-Proteobacteria | Desulfobacterium indolicum (sulfate reduction) | 91 | 5 | 202 | ||
| January | S1 | S1Jan1 | AJ877934 | β-Proteobacteria | Gallionella ferruginea (iron oxidation) | 97 | 66 | 217 |
| S1Jan50 | AJ877949 | δ-Proteobacteria | Desulfobacterium indolicum (sulfate reduction) | 93 | 5 | 202 | ||
| COWG | COWGJan9 | AJ877935 | β-Proteobacteria | Gallionella ferruginea (iron oxidation) | 95 | 70 | 217 | |
| COWGJan29 | AJ877957 | β-Proteobacteria | Thiobacillus plumbophilus (sulfide oxidation) | 91 | 5 | 219 | ||
| COWGJan20 | AJ877953 | γ-Proteobacteria | A. ferrooxidans (sulfide/iron oxidation and iron reduction) | 95 | 4 | 250-197 | ||
| COWGJan58 | AJ877951 | δ-Proteobacteria | Desulfomonile tiedjei (sulfate reduction) | 91 | 1 | 202 |
a
Corresponds to the relative abundance of clones for each library.
b
T-RFs correspond to expected terminal fragments with HaeIII predictive digestion.
c
The phylogenetic group, closest relatives, and postulated metabolism and relative abundance of clones are indicated.