TABLE 4.
Enumeration of Prymnesium sp. in natural samples by IFA labeling and detection by the SPC or by direct microscopic counting
| Method | Sample | Fixative | No. of fluorescent eventsa | No. of algaeb | Mean PI (SD) | Concn of algae (103 cells ml−1) |
|---|---|---|---|---|---|---|
| SPC | Shore | Glutaraldehyde | 620.7 | 578.7 | 1,346.7 (347.0) | 11.3-11.9 |
| Formaldehyde | 406.0 | 390.0 | 883.3 (229.0) | 7.4-8.2 | ||
| Offshore | Glutaraldehyde | 579.0 | 559.3 | 1,096.7 (218.0) | 9.8-12.6 | |
| Microscopic counting | Shore | 10.6-12.2 | ||||
| Offshore | 7.8-10.2 |
a
Number of fluorescent events after discrimination (means for three membranes).
b
Number of events validated as algae by microscopy (means for three membranes).