TABLE 2.
Performance of real-time fluorogenic RT-PCR assays for detection of bacteriophage MS2
| Assaya | Avg CT at sample concnb:
|
||||
|---|---|---|---|---|---|
| 4 pg | 0.4 pg | 40 fg | 4 fg | 0.4 fg | |
| 1 | 20.9 ± 0.4 | 24.5 ± 0.4 | 27.9 ± 0.2 | 33.3 ± 0.5 | ND |
| 2 | 23.8 ± 0.6 | 27.3 ± 0.6 | 32.4 ± 1.3 | ND | ND |
| 3 | 27.4 ± 0.4 | 31.0 ± 0.4 | 36.3 ± 1.3 | ND | ND |
| 4 | 24.6 ± 0.3 | 28.2 ± 0.2 | 31.8 ± 0.1 | 35.5 ± 0.2 | 44.5 ± 1.0 |
| 5 | 26.3 ± 0.5 | 29.9 ± 0.5 | 33.4 ± 0.2 | 37.0 ± 0.8 | 41.0 ± 2.6 |
a
Assays are numbered as in Table 1.
b
CT represents the cycle number at which fluorescence increases above the threshold dividing positive from negative results. The values shown are the average result from eight wells (four wells per experiment, two experiments) ± standard deviation. The concentrations reflect the amount of MS2 RNA per 20-μl reaction well. ND, no detection above threshold before cycle 45.