TABLE 4.

Resolution of real-time fluorogenic RT-PCR assay 1 for quantification of bacteriophage MS2 RNA^a

Sample concn (pg)	Avg CTb
400	13.3 ± 0.16
200	14.2 ± 0.14
100	15.2 ± 0.17
50	16.2 ± 0.15
25	17.2 ± 0.13
12.5	18.1 ± 0.13
6.25	19.2 ± 0.12
3.125	20.1 ± 0.09
1.5625	21.3 ± 0.16

^aThe probe and primer set used in this experiment were those of assay 1 (Table 1).

^bC_T represents the cycle number at which fluorescence increases above the threshold dividing positive from negative results. The values shown are the average result from eight wells (four wells per experiment, two experiments) ± standard deviation. The concentrations reflect the amount of MS2 RNA per 20-μl reaction volume.