FIG. 2.

Effects of the amino acids alanine (1 mM), cysteine (1 mM), threonine (1 mM), and glutamine (1 mM) on the germination of spores of the wild-type strain and the gerR and gerG mutant strains. Germination was monitored as the fall in OD₆₀₀ over 60 min. ▾, wild-type strain with 1 mM cysteine; •, wild-type strain with 1 mM alanine; ▴, wild-type strain with 1 mM threonine; ▪, wild-type strain with 1 mM glutamine; ○, gerR strain with 1 mM alanine; □, gerG strain with 1 mM glutamine; ⋄, wild-type strain with no germinant. Germination data for gerR spore responses to cysteine and threonine were identical to those for the response to alanine and were omitted for clarity. Germination data for the gerR, gerQ, gerK, gerL, gerS, and gerI mutant strains upon the addition of l-glutamine were similar to those for the wild type and were omitted for clarity.