FIG. 2.

RT-PCR analysis of avrPtoB homolog transcripts. Total RNA was isolated from each Pseudomonas strain after growth without (lanes −) or with (lanes +) Hrp induction. RT-PCR was performed as described in Materials and Methods using gene-specific primers; primers for 16S rRNA were used as an internal control. The PCR products were separated on an agarose gel and stained with ethidium bromide. Transcripts for each of the AvrPtoB homologs could be detected in the strain from which the corresponding gene was isolated.