TABLE 2.
Primers used to amplify avrPtoB homologs in different assays
| Primer | Sequence (5′→3′) | Nucleotide positiona |
|---|---|---|
| avrPto2-30 | ATGGCGGGTATCAA(C/T)(A/G)GAGC(A/G)G | 1-22 |
| avrPto2-3 | GACCTTCGAAGTGGCAGTGA | |
| avrPtoB-hrp | GGTGTGATGGAACTCTTTCCTGCTCb | |
| avrPtoBseq-1 | TATCGTTCAGCAATTGGTCAGTG | 393-415 |
| avrPtoBseq-2 | CCCCGGGTTCAGGTTAA | 1230-1224 |
| avrPtoBH-F | ATGGCGGGTATCAATAGAGCGG | 1-22 |
| avrPtoBH-R | TCAGGGGACTATTCTAAAAGC | 1740-1720 |
| avrPtoBHRT-F | GCCACGCGATAGCTCTTCCTTCTC | 509-532 |
| avrPtoBHRT-R | AACAACCGCCTGCCGCTCGTAAC | 1377-1355 |
| B728a-hrp | ACGCATCAGTGGAACCTTTb | |
| B728a-F | ATGTCGGGTATCAACGGAGCAG | 1-22 |
| B728a-R | TCAGTCAGGAACGACTCTAAAG | 1551-1530 |
| B728aseq-1 | ATGCCTCAGAAATGTAATGGATAA | 452-464 |
| B728aseq-2 | CTCCGCTTCAGGGTTCAG | 1214-1241 |
| hopPmaL-hrp | GATTTGCCGGGAGAAGTAAGGc | |
| hopPmaL-F | ATGGTAGGGATCAGTGGTAGAGCAG | 1-25 |
| hopPmaL-R | TCAAAATGCCTATGCATCAAGCC | 1158-1145 |
| hopPmaL-RT-R | GGCGAACCGGCCTAATAAT | 1029-1011 |
| 16SrRNA-F | GCGGCAGGCCTAACACAT | |
| 16SrRNA-R | GTTCCCCTACGGCTACCTT | |
| GWApBF | GGGGACAAGTTTGTACAAAAAAGCAGGCTTTGAGAATCTTTATTTTCAGGGCATGGCGGGTATCAATAGAGC | |
| GWApBR | GGGGACCACTTTGTACAAGAAAGCTGGGTTTCAGGGGACTATTCTAAAAGC |
a
Nucleotide position of avrPtoB or avrPtoB homolog.
b
The hrp box is underlined.
c
Upstream of hrp box.