TABLE 1.

Steady-state kinetic parameters for wild-type Cab and Cab variants with TAPS buffer (pH 8.5)^a

Variant	kcat (104 s−1)	Km in CO2 (mM)	kcat/Km (106 s−1 M−1)
Wild type	2.4 ± 0.2	2.9 ± 0.3	8.3 ± 0.7
D34A	0.42 ± 0.01	3.6 ± 0.4	1.2 ± 0.3
D34E	0.36 ± 0.1	1.8 ± 0.2	2.0 ± 0.5
R36A	0.0046 ± 0.0008	36.2 ± 1.5	0.0013 ± 0.0005
R36K	0.0092 ± 0.0007	7.2 ± 0.2	0.013 ± 0.004
D34A-R36A	0.052 ± 0.0006	9.2 ± 0.8	0.56 ± 0.03

^aValues are means ± standard errors.