TABLE 1.
Geographical origin of stone samples used in this study, detection of Geodermatophilaceae using primers Geosp2 and Geosp1, and concentration of total DNA
Sample | Geographical origin | Stone type | Total DNAb | RT-PCR amplificationa (Geosp2/Geosp1) |
---|---|---|---|---|
1A | La Cabrera (Madrid) | Granite (stone) | 5.69 | 1 |
1B | La Cabrera (Madrid) | Granite (lichen) | 15.17 | 1 |
2A | La Cabrera (Madrid) | Granite (stone) | 29.58 | 1 |
2B | La Cabrera (Madrid) | Granite (lichen) | 59.28 | 1 |
3 | Cala S. Vicente (Mallorca) | Limestone | 9.40 | 0 |
4 | Cala S. Vicente (Mallorca) | Limestone | 69.99 | 1 |
5A | El Atazar (Madrid) | Slate (stone) | 41.99 | 1 |
5B | El Atazar (Madrid) | Slate (lichen) | 89.21 | 1 |
Amplification results: 1, positive amplification; 0, absence of amplification products.
Values are expressed as ng of DNA/mg of stone.