TABLE 2.
RT-PCR quantification of Geodermatophilaceae DNA in serial dilutions of stone DNA samples using the primers Geosp2 and Geosp1a
| Sample | DNA quantification (ng/μl)
|
|||||
|---|---|---|---|---|---|---|
| Group A at dilution:
|
Group B at dilution:
|
|||||
| 10−1 | 10−2 | 10−3 | 10−1 | 10−2 | 10−3 | |
| 1A (stone) | 2.18 × 10−3 | 7.50 × 10−4 | NA | 3.22 × 10−3 | 3.43 × 10−4 | NA |
| 1B (lichen) | 9.70 × 10−3 | 9.52 × 10−5 | NA | 6.24 × 10−3 | 1.17 × 10−4 | NA |
| 2A (stone) | 1.66 × 10−2 | 8.40 × 10−4 | NA | 7.06 × 10−3 | 6.27 × 10−4 | NA |
| 2B (lichen) | 3.40 × 10−3 | 1.18 × 10−4 | NA | 3.25 × 10−3 | 8.42 × 10−5 | NA |
| 3 (limestone) | NA | NA | NA | NA | NA | NA |
| 4 (limestone) | 7.78 × 10−3 | 1.31 × 10−4 | NA | 3.74 × 10−3 | 4.55 × 10−4 | NA |
| 5A (stone) | 1.34 × 10−3 | NA | NA | 2.49 × 10−3 | NA | NA |
| 5B (lichen) | 1.46 × 10−3 | NA | NA | 1.17 × 10−3 | NA | NA |
a
Standard curves were established using serial dilutions of DNA from Geodermatophilus obscurus DSM43160T (group A) and DNA from Blastococcus aggregatus DSM4725T (group B). NA, no amplification.