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Fungal FISH analysis of the mucus biofilm. Four-micrometer sections of SPF mouse cecum were hybridized with a fungal small-subunit rRNA probe (PF2, 5′-FITC) and counterstained with DAPI. Magnifications: (a) ×200; (b to e) ×1,000; (f and g) ×2,500. For panel g, a white line was added to the FISH image to highlight the outline of this structure.
