TABLE 4.

Detection of rRNA genes^a by sequence-selective PCR amplification of DNA extracted from six different mouse chows

Taxon	PCR products from mouse chowsb
	RF	SPF	Mouse diet, no. 5015	Mouse diet 9F, no. 5020	Autoclavable mouse breeder diet, no. 5021	PicoLab mouse diet 20, no. 5058
Firmicutes	+	+c	+c	+c	+c	+c
Filobasidium	−/+	+	+	+	+	+
Lactobacillus	+	−	−/+	+	−	−/+
Smittium	−/+	−/+	−/+	+	+	+

^aFour of the largest assemblages of rRNA gene clones identified by the OFRG analyses of murine intestine (Tables 1 and 2).

^bThe presence or absence of PCR products is indicated as follows: +, product was detected; −, product was not detected; −/+, detection of product was variable in replicate experiments. RF designates the type of Harlan Teklad (Madison, Wisconsin) diet fed to RF mice, autoclaved sterilizable rodent diet (W) (catalog no. 8656); SPF designates the diet fed to SPF mice, rodent diet (W) (catalog no. 8604). The four other diets are different types of PMI LabDiet chow.

^cPCR products were produced but had high identity to corn mitochondrial rRNA genes.