TABLE 4.
Conjugational recombination with different ΔruvABC recipient strains
| Recipient strain | Relevant genotypea | Relative viabilityb | Avg relative yield of recombinantsc ± SD |
|---|---|---|---|
| AB1157 | Wild type | 1 | 1 |
| LMM10 | ΔruvABC | 0.62 ± 0.02 | 0.3 ± 0.12 |
| JC5519 | recBC | 0.29 ± 0.03 | 0.0032 ± 0.0013 |
| LMM20 | recBC ΔruvABC | 0.25 ± 0.04 | 0.0026 ± 0.0007 |
| JC7623 | recBC sbcBC | 0.59 ± 0.07 | 1 |
| LMM864 | recBC sbcBC ΔruvABC | 0.14 ± 0.01 | 0.022 ± 0.007 |
| LMM965 | sbcBC | 0.72 ± 0.08 | 1 |
| LMM966 | sbcBC ΔruvABC | 0.21 ± 0.03 | 0.063 ± 0.004 |
| LMM979 | sbcB | 0.92 ± 0.09 | 0.77 ± 0.18 |
| LMM983 | sbcB ΔruvABC | 0.25 ± 0.03 | 0.13 ± 0.02 |
| N2364 | sbcC | 1 | 1 |
| LMM971 | sbcC ΔruvABC | 0.68 ± 0.11 | 0.24 ± 0.05 |
a
In all strains listed, the designation sbcB corresponds to the sbcB15 mutation.
b
Viability is given relative to the number of CFU per milliliter in the cultures of the control recipient strain AB1157, which averaged 5.7 × 107.
c
Yields of recombinants are relative to the control strain AB1157 and have been corrected for any deficiency in the viability of the recipient cells. The average yield for the control strain AB1157 was 6.4 × 105 per ml of the mating mixture. Values are averages ± standard deviations of results from three independent experiments.