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SDS-polyacrylamide (12.5%) gel analysis of various samples using PhastSystem (Amersham Pharmacia Biotech) during QAPRTase protein purification from overexpression of nadC in E. coli. The gel was stained with Coomassie blue. Lane 1, low-molecular-weight protein marker; lane 2, crude proteins; lane 3, purified GST-QAPRTase fusion protein; lane 4, fusion protein cleaved by protease factor Xa; lane 5, purified QAPRTase protein.
