TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Pseudomonas strains | ||
| PsJN | Wild type, growth-promoting activity | 27 |
| PsJN(Rifr) | Spontaneous Rifr derivative of PsJN | This study |
| H41 | Mutant, non growth-promoting activity, Rifr, Kmr | This study |
| E. coli strains | ||
| DH5α | F−recA1 endA1 gyrA1 thi-1 hsdR17 supE44 relA1 Φ80dlacIqZΔM15 λ− Δ(lacZ-argF)U169 | Stratagene |
| XL1-Blue | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 λ−lac [F′proAB lacIqZΔM15 Tn10(tet)] | Stratagene |
| BL21 | F−ompT hsdS (rB− mB−) gal | Amersham |
| LE392 | F−hsdR514 supE44 supF58 lacY1 galK2 galT22 metB1 trpR55 λ− | Promega |
| WA803 | met thi | 46 |
| Plasmids | ||
| pGS9 | Suicide vector, Cmr, Tn5, Kmr | 41 |
| pBluescript | pBluescript II SK(+) cloning vector, Apr | Stratagene |
| pRK415 | Inc PI (RK2 replicon), Tcr, plac and polylinker of pUC19 | 20 |
| pGEX-5X-3 | GST fusion protein expression vector, Apr, lacIq, factor Xa cleavage site | Amersham |