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. 2006 Jan;72(1):869–879. doi: 10.1128/AEM.72.1.869-879.2006

TABLE 1.

Summary of the RNase H cleavage assays

Site Position in EF-Tu mRNA mRNA sequencea RNase H cleavage
1 55-61 5′-cGGACACG-3′ +
2 130-136 5′-aGCGACUG-3′ +
3 154-160 5′-uGCUGCUC-3′ NSb
4 196-202 5′-uGCUCACA-3′ +
5 232-238 5′-uGCCCACA-3′ ++
6 244-250 5′-cGCUCCAG-3′ NS
7 250-256 5′-aGGUCACG-3′ +
8 256-262 5′-cGCGGACU-3′ +
9 286-292 5′-uGGCUGCC-3′ ++
a

The lowercase nucleotides are at position-1 in reference to the cleavage site. The uppercase nucleotides base pair with δRz to form the P1 stem (Fig. 1B).

b

NS, nonspecific cleavage was observed.