TABLE 1.
Summary of the RNase H cleavage assays
| Site | Position in EF-Tu mRNA | mRNA sequencea | RNase H cleavage |
|---|---|---|---|
| 1 | 55-61 | 5′-cGGACACG-3′ | + |
| 2 | 130-136 | 5′-aGCGACUG-3′ | + |
| 3 | 154-160 | 5′-uGCUGCUC-3′ | NSb |
| 4 | 196-202 | 5′-uGCUCACA-3′ | + |
| 5 | 232-238 | 5′-uGCCCACA-3′ | ++ |
| 6 | 244-250 | 5′-cGCUCCAG-3′ | NS |
| 7 | 250-256 | 5′-aGGUCACG-3′ | + |
| 8 | 256-262 | 5′-cGCGGACU-3′ | + |
| 9 | 286-292 | 5′-uGGCUGCC-3′ | ++ |
a
The lowercase nucleotides are at position-1 in reference to the cleavage site. The uppercase nucleotides base pair with δRz to form the P1 stem (Fig. 1B).
b
NS, nonspecific cleavage was observed.