TABLE 3.
BSR of P. putida KT2440 at different growth phasesa
| Lyoprotectant | Mean BSR (SD) at:
|
||
|---|---|---|---|
| Exponential (early) phase | Exponential (late) phase | Late stationary phase | |
| Myoinositol | 12.21 (8.00) | 22.55 (7.5) | 47.18 (2.00) |
| Trehalose | 62.44 (5.0) | 78.46 (5.0) | 90.15 (1.50) |
a
P. putida KT2440 was grown on LB overnight. The culture was then diluted 100-fold. When it reached a turbidity at 660 nm of about 0.6 (early exponential phase), 1.2 (late exponential phase), or 4 (late stationary phase), aliquots were lyophilized with myoinositol or trehalose as the lyoprotectant.