TABLE 1.
Oligoprimers used for PCR amplification of cmmGI, cmmGII, cmmGIII, and cmmGIV
Gene | Primer | Nucleotide sequence (5′-3′)a |
---|---|---|
cmmGI | Forward | GGACTAGTCCCACGGAGTTTCCTATG |
Reverse | GCTCTAGAGTTAACTCAGTCTCCG GTGTCCG | |
cmmGII | Forward | GGACTAGTGCTAGCGGAGCCCTCG CCTGTCATG |
Reverse | GCTCTAGACTAACCGGCGCATCGCCG | |
cmmGIII | Forward | GGACTAGTGTTAACGTGGAGAGGT GAGTGGCATG |
Reverse | GCTCTAGACCTAGGTCAGGGCCCG GTCCTGGC | |
cmmGIV | Forward | GGACTAGTCCTAGGCTCTGGGTGC CACTGAAAG |
Reverse | GCTCTAGAGCTAGCTCATCCTGCC TCTTTCGTC |
Sequences for restriction sites are underlined. SpeI, 5′-ACTAGT-3′; XbaI, 5′-TCTAGA-3′; HpaI, 5′-GTTAAC-3′; NheI, 5′-GCTAGC-3′; AvrII, 5′-CCTAGG-3′.