FIG. 3.

Analysis of IS1071-mediated cointegrates by agarose gel electrophoresis (A) and Southern hybridization patterns with an internal 1.1-kb fragment of IS1071 (Fig. 1C) as a probe (B). All samples were digested with SalI, which cleaved IS1071 at one site (Fig. 1C). Lane 1, marker DNA; lane 2, pMS0252; lanes 3 to 6, cointegrates of pMS0252 and R388 obtained in the independent experiments; lane 7, R388. The sizes (in kilobases) of three fragments in the marker DNA are shown on the left.