TABLE 2.
Transposition of IS1071
| Host straina | Proteobacterial division | Transposition frequencyb
|
|
|---|---|---|---|
| pBBR1MCS-3 | pMS0252 | ||
| A. tumefaciens C58 | α | <1.2 × 10−7 | <8.7 × 10−8 |
| C. testosteroni JCM5832 | β | <3.3 × 10−7 | 1.5 × 10−3 |
| D. acidovorans B123c | β | <1.4 × 10−5 | 4.0 × 10−4 |
| E. coli HB101 | γ | <9.8 × 10−9 | <1.6 × 10−8 |
| P. alcaligenes JCM5967 | γ | <9.7 × 10−8 | <2.1 × 10−8 |
| P. putida PpN1 | γ | <1.9 × 10−8 | <1.4 × 10−8 |
a
D. acidovorans B123 carried more than one copy of IS1071 in its chromosome, and the other strains were free of IS1071 (data not shown).
b
Transposition of IS1071 was detected by the cointegration between pMS0252 and R388 (32). Plasmid pMS0252 is a pBBR1MCS-3 derivative carrying IS1071 (Fig. 1C). The transposition frequency is expressed as the number of Tcr Nalr transconjugants per number of Sur Nalr transconjugants. A value with the symbol< means that no Tcr Nalr transconjugants were obtained in the experiment. All values are averages from at least three independent experiments.
c
The transfer frequency of R388 was low (2 × 10−4 per donor cell) for an unknown reason(s) when D. acidovorans B123 was used as the donor host.