TABLE 3.
Transposition of Kmr-IS1071 derivatives with mutant IRsa
| Expt | Donor of Kmr-IS1071b (IR length, bp) | Transposition frequencyc
|
|
|---|---|---|---|
| pBBR1MCS | pMS0311 | ||
| 1 | pMS0310 (110) | 8.5 × 10−7 | 3.7 × 10−3 |
| 2 | PMS0366 (100) | 1.5 × 10−6 | 8.7 × 10−6 |
| 3 | pMS0364 (90) | 2.4 × 10−6 | 6.0 × 10−6 |
| 4 | pMS0363 (70) | 8.0 × 10−7 | 4.2 × 10−7 |
| 5 | pMS0362 (48) | 8.7 × 10−7 | 3.8 × 10−6 |
| 6 | pMS0361 (38) | 4.7 × 10−7 | 2.9 × 10−6 |
| 7 | pMS0368 (90, Δ51-70) | 3.7 × 10−6 | 8.7 × 10−6 |
| 8 | pMS0369 (90, Δ71-90) | 3.1 × 10−6 | 2.8 × 10−6 |
a
The donor strain was a C. testosteroni JCM5832 derivative harboring the three following plasmids: (i) R388, (ii) a pNIT6012 derivative carrying a Kmr-IS1071 derivative with the mutant IRs, and (iii) either pBBR1MCS or pMS0311, a pBBR1MCS derivative that carried the tnpA gene of IS1071 (Fig. 1B and C).
b
The IR sequences and structure, respectively, of each Kmr-IS1071 derivative are shown in Fig. 1B and C.
c
The transposition frequency is expressed as the number of Kmr Nalr transconjugants per number of Sur Nalr transconjugants. All values are averages from at least three independent experiments.