TABLE 4.
Promoter analysis of the tnpA genea
| Host strain | IS1071-mediated cointegration | β-Gal activity (Miller units)
|
|
|---|---|---|---|
| pMS0324 | pMS0326 | ||
| A. tumefaciens C58 | No | 142 ± 31 | 7.6 ± 2.1 |
| C. testosteroni JCM5832 | Yes | 9.2 ± 0.6 | NDb |
| D. acidovorans B123 | Yes | ND | ND |
| E. coli DH5α | No | ND | ND |
| P. alcaligenes JCM5967 | No | ND | ND |
| P. putida PpN1 | No | 33 ± 4.0 | ND |
a
β-Gal activity was assayed by the method of Miller (19). Plasmid pMS0324 carries a tnpA promoter-lacZ transcriptional fusion, whereas pMS0326 lacks the tnpA promoter region and was used as a negative control. The nucleotide sequence upstream of the tnpA gene is shown in Fig. 2A, and the structures of the plasmids used in this analysis are shown in Fig. 2B. The values are averages from three independent experiments and are shown with the standard deviations.
b
ND, not detected in a 60-min reaction.