TABLE 1.
Strain or plasmid | Relevant characteristicsd | Source or reference |
---|---|---|
Laboratory strains | ||
E. coli TOP10 | Electrocompetent or chemically competent; F−mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 (ara-leu)7697 galUgalK rpsL (Strr) endA1 nupG; Gens (MIC, <3.9 μg/ml) | Invitrogen |
E. coli strain BM14 | Azir; Gens (MIC, <3.9 μg/ml) | 8 |
Environmental strains | ||
S. enterica subsp. enterica, strain S5 | Genr Kanr Tobr; GEN MIC, >2,000 μg/ml | Turtle nest eggsa |
S. enterica subsp. arizonae, strain A40 | Genr Kanr Tobr; GEN MIC, >2,000 μg/ml | Infected turtlesb |
S. enterica subsp. arizonae, strain PH5 | Genr Kanr Tobr; GEN MIC, >2,000 μg/ml | Turtle pond waterc |
E. cloacae, strain E19 | Genr Kanr Tobr; GEN MIC, >2,000 μg/ml | Infected turtlesb |
C. freundii, strain C14 | Genr Kanr Tobr; GEN MIC, >2,000 μg/ml | Turtle nest eggsa |
Plasmids | ||
pGEM-7Zf | Cloning and sequencing vector; Ampr | Promega |
pCR2.1-TOPO | Cloning and sequencing vector; Kanr | Invitrogen |
pSal5 | R plasmid isolated from S. enterica subsp. enterica, strain S5 | This study |
pAri40 | R plasmid isolated from S. enterica arizonae, strain A40 | This study |
pAriPH5 | R plasmid isolated from S. enterica subsp. arizonae, strain PH5 | This study |
pEnt19 | R plasmid isolated from E. cloacae, strain E19 | This study |
pCit14 | R plasmid isolated from C. freundii, strain C14 | This study |
pGSal5h | pGEM-7Zf derivative containing a 5.5-kb HindIII fragment from pSal5 | This study |
pGEnt19b | pGEM-7Zf derivative containing a 5.0-kb BamHI fragment from pEnt19 | This study |
Supplied by Central Analytical Laboratory, Belle Chase, LA, July 1999.
Isolated from turtle breeding pond water in Louisiana, June 2000.
Abbreviations: Ampr, ampicillin resistant; Azir, sodium azide resistant; Genr, gentamicin resistant; Gens, gentamicin sensitive; Kanr, kanamycin resistant; Tobr, tobramycin resistant.