TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristicsd	Source or reference
Laboratory strains
E. coli TOP10	Electrocompetent or chemically competent; F−mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 (ara-leu)7697 galUgalK rpsL (Strr) endA1 nupG; Gens (MIC, <3.9 μg/ml)	Invitrogen
E. coli strain BM14	Azir; Gens (MIC, <3.9 μg/ml)	8
Environmental strains
S. enterica subsp. enterica, strain S5	Genr Kanr Tobr; GEN MIC, >2,000 μg/ml	Turtle nest eggsa
S. enterica subsp. arizonae, strain A40	Genr Kanr Tobr; GEN MIC, >2,000 μg/ml	Infected turtlesb
S. enterica subsp. arizonae, strain PH5	Genr Kanr Tobr; GEN MIC, >2,000 μg/ml	Turtle pond waterc
E. cloacae, strain E19	Genr Kanr Tobr; GEN MIC, >2,000 μg/ml	Infected turtlesb
C. freundii, strain C14	Genr Kanr Tobr; GEN MIC, >2,000 μg/ml	Turtle nest eggsa
Plasmids
pGEM-7Zf	Cloning and sequencing vector; Ampr	Promega
pCR2.1-TOPO	Cloning and sequencing vector; Kanr	Invitrogen
pSal5	R plasmid isolated from S. enterica subsp. enterica, strain S5	This study
pAri40	R plasmid isolated from S. enterica arizonae, strain A40	This study
pAriPH5	R plasmid isolated from S. enterica subsp. arizonae, strain PH5	This study
pEnt19	R plasmid isolated from E. cloacae, strain E19	This study
pCit14	R plasmid isolated from C. freundii, strain C14	This study
pGSal5h	pGEM-7Zf derivative containing a 5.5-kb HindIII fragment from pSal5	This study
pGEnt19b	pGEM-7Zf derivative containing a 5.0-kb BamHI fragment from pEnt19	This study

^aIsolated from nest egg homogenate (26, 37, 38) at the Louisiana turtle farms, July 1999.

^bSupplied by Central Analytical Laboratory, Belle Chase, LA, July 1999.

^cIsolated from turtle breeding pond water in Louisiana, June 2000.

^dAbbreviations: Amp^r, ampicillin resistant; Azi^r, sodium azide resistant; Gen^r, gentamicin resistant; Gen^s, gentamicin sensitive; Kan^r, kanamycin resistant; Tob^r, tobramycin resistant.