TABLE 4.
R plasmids isolated from bacteria recovered from turtle farms
| Plasmid | Size (kb) | RFLPa type | Resistance phenotypeb | GEN MIC (μg/ml)b | Presence of GEN resistance and integron-associated genec
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| aac(3)-IIa | aac(3)-VIa | armA | rmtA | rmtB | intI1 | sulI | |||||
| pSal5 | ca. 45 | A | Genr Kanr Tobr | >2,000 | + | − | − | − | − | − | + |
| pAri40 | ca. 145 | B | Genr Kanr Tobr | >2,000 | − | + | − | − | − | + | + |
| pAriPH5 | ca. 145 | B | Genr Kanr Tobr | >2,000 | − | + | − | − | − | + | + |
| pEnt19 | ca. 115 | C | Genr Kanr Tobr | >2,000 | − | + | − | − | − | + | + |
| pCit14 | ca. 45 | D | Genr Kanr Tobr | >2,000 | + | − | − | − | − | − | − |
a
RFLP, restriction fragment length polymorphism.
b
Determined using transformant TOP10 E. coli cells.
c
As determined by PCR and/or sequencing.