TABLE 3.
Subgroup C genes showing differences in temporal expression in response to strontium stress
| Gene | Gene product | Mean ratio (SrCl2/control) ata:
|
|||
|---|---|---|---|---|---|
| 5 min | 30 min | 60 min | 90 min | ||
| SO3599 | Sulfate ABC transporter, periplasmic sulfate-binding protein (CysP) | 1.2 | 3.5 | 3.5 | 0.4 |
| SO3723 | Adenylylsulfate kinase (CysC) | 1.2 | 3.6 | 3.8 | 0.4 |
| SO3724 | Hypothetical protein | 1.1 | 3.4 | 4.3 | 0.3 |
| SO3725 | Hypothetical protein | 1.1 | 3.8 | 3.8 | 0.4 |
| SO3726 | Sulfate adenylyltransferase, subunit 1 (CysN) | 0.9 | 2.7 | 3.4 | 0.3 |
| SO3727 | Sulfate adenylyltransferase, subunit 2 (CysD) | 0.9 | 3.3 | 3.4 | 0.4 |
| SO3736 | Phosphoadenosine phosphosulfate reductase (CysH) | 0.8 | 3.3 | 3.2 | 0.3 |
| SO3737 | Sulfite reductase (NADPH) hemoprotein beta-component (CysI) | 1.0 | 3.8 | 3.3 | 0.3 |
| SO3738 | Sulfite reductase (NADPH) flavoprotein alpha-component (CysJ) | 1.0 | 3.3 | 3.6 | 0.4 |
| SO3819 | Nitrogen regulatory protein, P-II | 0.4 | 1.2 | 0.9 | 0.08 |
| SO4026 | Hypothetical protein | 0.6 | 2.4 | 7.7 | 0.2 |
| SO4410 | Glutamine synthetase, type 1 (GlnA) | 0.3 | 1.4 | 0.8 | 0.06 |
| SO4654 | Sulfate ABC transporter, permease protein (CysW-2) | 0.8 | 2.8 | 5.9 | 0.6 |
| SO4655 | Sulfate ABC transporter, ATP-binding protein (CysA-2) | 0.9 | 3.5 | 6.9 | 0.5 |
a
Relative gene expression is presented as the mean ratio of the fluorescence intensity of SrCl2-exposed cells to that of control cells. Each gene showed significant differential expression (P < 0.05) at a minimum of one time point. The time in minutes is that at which cells were harvested for RNA isolation following addition of 180 mM SrCl2 to the experimental culture.