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. 2002 Aug;184(15):4104–4113. doi: 10.1128/JB.184.15.4104-4113.2002

TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid Relevant genotype and/or phenotype Source or reference
Strains
    E. coli
        DH5αλpir F− Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 λ::pir 10, 14
        Sm10λpir thi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pir Laboratory strain
        AC-E479 Sm10λpir(pSL479) Apr 16
        AC-E481 Sm10λpir(pSL481) Apr 16
        AC-E482 Sm10λpir(pSL482) Apr This work
        AC-E1078 Sm10λpir(pSL148) Apr This work
        AC-E1082 Sm10λpir(pSL108) Apr This work
        AC-E1211 Sm10λpir(pAT1211) Apr This work
    V. cholerae El Tor biotype
        AC-V66 C6709-1 lacZ::res-tet-res Smr Tcr 2
        Bah-2 E7946 ΔattRS SmrlacZ 22
        AC-V282 C6709-1 ΔvieSAB Smr Tcr 17
        AC-V279 AC-V66 ΔvieAB Smr Tcr 17
        AC-V494 AC-V66 ΔtoxT Smr Tcr 16
        AC-V752 AC-V66 vieS::mTn5Kn2 Smr Tcr Knr 18
        AC-V765 AC-V66 ΔvieSAB Smr Tcr This work
        AC-V323b AC-V66 ΔvieB Smr Tcr This work
        AC-V1079 AC-V66 ΔvieS Smr Tcr This work
        AC-V1221 AC-V66 ΔvieA-HTH Smr Tcr This work
        AC-V553 AC-V66 ctxA::tnpR135 Smr Tcr Apr 16
        AC-V1023 AC-V752 ctxA::tnpR135 Smr Tcr Apr 18
        AC-V1083 AC-V323b ctxA::tnpR135 Smr Tcr Apr This work
        AC-V1097 AC-V66 ctxA::tnpR168 Smr Tcr Apr This work
        AC-V1098 AC-V752 ctxA::tnpR168 Smr Tcr Apr This work
        AC-V1151 AC-V1079 ctxA::tnpR135 Smr Tcr Apr This work
        AC-V1093 AC-V765 tcpA::tnpR135 Smr Tcr Apr This work
        AC-V1117 AC-V66 pMMB67EH Smr Tcr Apr This work
        AC-V1110 AC-V765 pMMB67EH Smr Tcr Apr This work
        AC-V1118 AC-V66 pAT1114 Smr Tcr Apr This work
        AC-V1120 AC-V765 pAT1114 Smr Tcr Apr This work
Plasmids
    pIVET5 oriR6K mobRP4 tnpR-lacZY Apr 2
    pSL479 pIVET5::tcpA′::tnpR135 16
    pSL481 pIVET5::ctxA′::tnpR135 16
    pSL482 pIVET5::ctxA′::tnpR168 This work
    pCVD442 oriR6K mobRP4 sacB Apr 6
    pSL108 pCVD442::ΔvieB 17
    pSL148 pCVD442::ΔvieS This work
    pAT1211 pCVD442::ΔvieA-HTH This work
    pCR-Script f1(+) ori Apr Stratagene
    pMMB67EH IncQ broad-host-range cloning vector, Apr 21
    pAT1113 pCR-Script::vieSAB This work
    pAT1114 pMMB67EH::vieSAB This work
    pGEM-T f1 ori Apr Promega
    pAT853 pGEM-T::′ctxA′ This work
    pAT854 pGEM-T::′ctxB′ This work
    pAT856 pGEM-T::′rpoB′ This work
    pDSM701 pGEM-T::′toxT′ This work