TABLE 2.
Sequences of primers used in this study
| Primer | DNA sequence (5′→3′)a |
|---|---|
| CtxF1 | GCTCTAGATCGAGTCAGAGCAATCCGAG |
| CtxR1 | GAAGATCTGCATATGAAAATGATGATAA |
| VieSF4 | GCGAAAAGGAAATGGTATGAA |
| VieSR4 | AGCTGTTTTTTGATCATAATACTGCAATGGTGACTGT |
| VieSF5 | CACCATTGCAGTATTATGATCAAAAAAACAGCTCAGTG |
| VieSR5 | TGATACTGATGAAGCCACTGT |
| AHTHF1 | GCAGCTTTCCGTCAACATC |
| AHTHR1 | CTCTCGGTACTACTCTTCTTGTTGCGCCTGT |
| AHTHF2 | CAAGAAGAGTAGTACCGAGAGTTATGGCTG |
| AHTHR2 | TCCCCAACCGATAAGCAC |
| F18 | AATTGAGCAGTGTGGCTTGCAT |
| AHTHR3 | TGTTCCGATTCCGCTTGTC |
| F13 | TCGTCGTCAAAGCGAATACTT |
| R14 | CTGGTGCGGGCAGTCATAGAGT |
| F36 | CAACAACAGAGTGGTTTG |
| VieSR1 | ACATGCATGCTCTTAGCTTGAGCTTCAGGC |
| LSABF | TAGTCAGGTACCAGCAGTAATTGGACGAGCGAAAAGG |
| LSABR | TAGTCAGCATGCTTCAGAACAAAACCGAGGCAATG |
| CTAF | CTGTTAAACAAAGGGAGCATT |
| CTAR | GTGGGCACTTCTCAAACTAAT |
| CTBF | ATGCACATGGAACACCTCAA |
| CTBR | GCATGAGGCGTTTTATTATTC |
| RpoBF | CAGTTTGGGTCACTTATCAGC |
| RpoBR | ATTGGAAATACAGAACGAAAA |
| ToxTF | TTCAATTATCTTACTCAA |
| ToxTR | ACAAATATCTGCCCAACG |
| SP6 | GCACCCCAGGCTTTACAC |
| T7 | GGGTTTTCCCAGTCACGA |
a
Restriction enzyme sites are underlined.