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SDS-PAGE analysis of the fractions from Q-Sepharose chromatography of the crystal inclusion protein of the GP1 strain. (A) Protein profile of the spore-crystal mixture. (B) Elution profiles of anion-exchange chromatography with a linear gradient of 50 to 400 mM NaCl, in 50 mM Na2CO3 (pH 10.5), 0.2% β-mercaptoethanol. Lanes 1 to 8, fraction samples subjected to SDS-PAGE and stained with Coomassie brilliant blue; lane M, molecular masses (kDa) of standard marker proteins.
