TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Bacterial strains | ||
| Bifidobacterium longum | ||
| DJO10A | Wild-type human isolate; host of pDOJH10L and pDOJH10S | A. Islam, D. D. O’Sullivan, and D. J. O’Sullivan (unpublished) |
| VMK44 | Electroporation host | J. Steeleb |
| Lactobacillus delbrueckii subsp. bulgaricus | Electroporation host | L. L. McKay culture collection |
| E. coli | ||
| XL1-Blue | thi recA1 hsdR17 relA1 gyrA94 α-lacZ complementation; Tcr | 5 |
| Plasmids | ||
| pDOJH10L | 10-kb wild-type plasmid from B. longum DJO10A | This study |
| pDOJH10S | 3.6-kb wild-type plasmid from B. longum DJO10A | This study |
| pDOJHR | E. coli-bifidobacterium shuttle cloning vector; Cmr | This study |
| pUC18 | E. coli cloning vector; source of lacZ and MCS | 23 |
| pACYC184 | E. coli cloning vector; source of p15A ori | 6 |
| pCI372 | E. coli-Lactococcus lactis shuttle cloning vector; Cmr | 12 |
| pDOJ4 | E. coli-Lactobacillus bulgaricus shuttle-cloning vector; Cmr | J. Halgerson, J. H. Kim, J. H. Lee, and D. J. O’Sullivan (unpublished) |
a
Tcr, tetracycline resistance; Cmr, chloramphenicol resistance; ori, origin of replication; MCS, multiple cloning site.
b
Department of Food Science, University of Wisconsin.