FIG. 1.

Growth of T. maritima in pure culture and in coculture with M. jannaschii. (A) Epifluorescence micrographs of pure culture and coculture corresponding to the growth curves are shown from left to right. After inoculation, the pure culture grew without aggregating to a density of above 10⁸ cells/ml before entering a prolonged stationary phase, during which time the T. maritima cell morphology changed from rods to cocci. The coculture began to aggregate once cell densities reached approximately 5 × 10⁸ cells/ml until entering stationary phase (∼10⁹ cells/ml). In stationary phase, cells detached from aggregates, and by 24 h they displayed coccoid morphology similar to what is seen in the pure culture during late stationary phase. (B). Growth curves for T. maritima grown in pure culture and in coculture with M. jannaschii. Error bars indicate standard deviations. (C) Volcano plots comparing expression profiles of pure T. maritima culture (top) and coculture of T. maritima with M. jannaschii (bottom) during the transition of growth phases from mid-log to early stationary phase. The x axis is the log₂ fold change from mid-log to early stationary phase, and the y axis is the −log₁₀ P value for the calculated fold change.