TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant feature(s) | Reference |
|---|---|---|
| Strains | ||
| L. lactis NZ9000 | MG1363 pepN::nisRK | 24 |
| L. lactis NZ9700 | Nisin producing, containing Tn5276 | 23 |
| Plasmids | ||
| pNZ8048 | Cmr, inducible expression vector containing the nisA promoter | 24 |
| pNZ-His6-ComK | Cmr, his6-comK under control of PnisA | 31a |
| pNZ-ComKΔC25 | Cmr, his6-comKΔC25 under control of PnisA | 31a |
| pG-wt | Eryr, contains wild-type common K-box of comG promoter | 31a |
| pORI::PctrA | Eryr; pORI13 carrying a 233-bp ctrA promoter fragment | 6 |
| pPywcC | Eryr, contains PywcC-lacZ fusion | This study |
| pPtig | Eryr, contains Ptig-lacZ fusion | This study |
| pPctrA | Eryr, contains PctrA-lacZ fusion | This study |