TABLE 2.
Top 30 upregulated genes for wtComK or ComKΔC25 expressiona
| Gene | Description | Ratio of transcription
|
K-box | |
|---|---|---|---|---|
| wtComK | ComKΔC25 | |||
| dacA | d-Alanyl-d-alanine carboxypeptidase | 9.8 | 5.5 | |
| orf1837 | Hypothetical protein | 8.8 | 11.2 | |
| orf258 | Hypothetical protein | 8.2 | 13.0 | |
| yriC | Hypothetical protein | 6.5 | 15.9 | Yes |
| ydaG | ABC transporter, ATP-binding and permease protein | 6.3 | 5.7 | |
| dnaG | DNA primase | 6.2 | 3.9 | Yes |
| yjaE | Hypothetical protein | 6.2 | 5.4 | |
| guaC | GMP reductase | 5.0 | 2.4 | Yes |
| cadA | Cadmium efflux ATPase | 4.9 | 3.7 | |
| yrbB | Hypothetical protein | 4.7 | 2.2 | |
| yhfA | Hypothetical protein | 4.7 | 2.6 | |
| ymfD | Integrase-recombinase | 4.7 | 4.2 | |
| yriD | Hypothetical protein | 4.6 | 4.7 | Yes |
| adhE | Alcohol-acetaldehyde dehydrogenase | 4.5 | 6.3 | |
| ydbA | ABC transporter, ATP-binding and permease protein | 4.3 | 4.7 | |
| yleF | Transcription regulator | 4.1 | 2.2 | |
| ycgD | Oxidoreductase | 4.0 | 3.5 | |
| vacB1 | RNase | 4.0 | 3.5 | |
| trxH | Thioredoxin, H-type | 3.8 | 2.0 | |
| orf1734 | Hypothetical protein | 3.7 | ||
| ytjD | Hypothetical protein | 3.7 | 6.0 | |
| yuiE | Hypothetical protein | 3.6 | ||
| busAA | OpuAA | 3.4 | 3.5 | Yes |
| ywjF | 3-Hydroxyisobutyrate dehydrogenase | 3.4 | ||
| purM | Phosphoribosylformylglycinamide cycloligase | 3.2 | ||
| yccL | Hypothetical protein | 3.1 | ||
| ypaD | Hypothetical protein | 3.1 | 6.6 | |
| yejC | Hypothetical protein | 3.1 | ||
| yxdB | Hypothetical protein | 3.1 | 2.4 | Yes |
| ycdG | Hypothetical protein | 3.1 | 16.0 | |
a
The list of genes is based on the results for a wtComK-producing strain. For each gene, the putative function and the ratio of transcription relative to L. lactis NZ9000(pNZ8048) is indicated for wtComK and, if regulated, for ComKΔC25. The presence of a K-box within 200 bp from the transcription start site is also indicated.