FIG. 1.

Overview of genes and enzymes involved in the degradation of chloroaromatics by R. eutropha JMP134. The enzymes catalyzing the indicated catabolic steps are TfdA, 2,4-D/α-ketoglutarate dioxygenase, TfdB, chlorophenol hydroxylase, TfdC, chlorocatechol dioxygenase, TfdD, chloromuconate cycloisomerase, TfdE, dienelactone hydrolase, and TfdF, maleylacetate reductase. The tfd genes in R. eutropha are localized on plasmid pJP4. Arrows, orientations of the tfd genes described thus far; black triangle, insertion element ISJP4.