TABLE 1.

Specific activities of catechol and chlorocatechol catabolic enzymes of 2,4-D- or 3-CB-grown cells of R. eutropha JMP134, JMP222(pBBR1M-I), and JMP222(pBBR1M-II)

Enzyme activity	Assay substrate	Sp act (U/g of protein)a for indicated strain/substrate
		JMP134(pJP4)/ 5 mM 2,4-D	JMP134(pJP4)/ 5 mM 3CB	JMP222(pBBR1M-I)/ 5 mM 3CB	JMP222(pBBR1M-II)/ 2 mM 3CB
(Chloro) catechol 1,2-dioxygenase	Catechol	430 ± 90	840 ± 120	550 ± 90	540 ± 90
	3-Chlorocatechol	420 ± 90	380 ± 80	540 ± 90	290 ± 70
	4-Chlorocatechol	410 ± 80	370 ± 80	520 ± 90	260 ± 50
(Chloro) muconate cycloisomerase	Muconate	<5	120 ± 20	<5	90 ± 30
	2-Chloromuconate	25 ± 5	15 ± 5	35 ± 10	<5
	3-Chloromuconate	270 ± 40	180 ± 40	360 ± 40	140 ± 50
Dienelactone hydrolase	cis-Dienelactone	1,050 ± 120	980 ± 110	1,200 ± 400	55 ± 15
Maleylacetate reductase	Maleylacetate	750 ± 170	510 ± 150	470 ± 150	1,520 ± 300

^aValues are averages of at least three independent experiments.