TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or phenotype | Amino acid exchange in ribosome protein S12 | Source and/or reference |
|---|---|---|---|
| Bacillus subtilis strains | |||
| 168 | trpC2 | Laboratory stock | |
| KO272 | trpC2 str-10 (rpsL5) Smr | K56Q | Spontaneous rpsL mutant; 10 |
| WL1 | trpC2 rpsL1 Smr | K56R | 14 |
| WL2 | trpC2 rpsL2 Smr | K56N | 14 |
| WL3 | trpC2 rpsL3 Smr | K56T | 14 |
| WL4 | trpC2 rpsL4 Smr | K56I | 14 |
| WL5 | trpC2 rpsL5 Smr | K56Q | KO272→168a |
| WL6 | trpC2 rpsL6 Smr | K56H | pKF19k-rpsL6→168 |
| WL9 | trpC2 rpsL9 Smr | P104S | 14 |
| WL10 | trpC2 rpsL10 Smr | G105W | PCR product→168 |
| WL15 | trpC2 rpsL15 Smr | K101E | pKF19k-rpsL15→168 |
| Plasmids | |||
| pKF19k-rpsL | Template for site-directed mutagenesis | 14 | |
| pKF19k-rpsL6 | pKF19k-rpsL containing the rpsL6 (K56H) mutation | This study | |
| pKF19k-rpsL15 | pKF19k-rpsL containing the rpsL15 (K101E) mutation | This study |
a
The strain to the right of the arrow was transformed with the chromosomal DNA, PCR product, or plasmid to the left of the arrow.