TABLE 1.
Relative abundances of phylogenetic groups in rice root incubations on day 28, based on frequencies of 16S rRNA or 16S rRNA genes in clone libraries and T-RFLP analysis
| Phylogenetic group (T-RF length, bp)a | Relative abundance (%)
|
|||||
|---|---|---|---|---|---|---|
| Control incubation (rRNA gene; n = 24)
|
CH3F incubation (rRNA gene; n = 35)
|
Control incubation (rRNA; n = 31)
|
||||
| Clone library | T-RFLPb | Clone library | T-RFLPb | Clone library | T-RFLPb | |
| MB (92) | 0.9 | 5.0 | 1.8 | |||
| MS (186) | 87.5 | 92.8 | 12.5 | 14.0 | 100.0 | 99.2 |
| RC-I (393) | 2.7 | 17.5 | 21.5 | |||
| RC-III (381) | 0.7 | 17.5 | 9.0 | 0.8 | ||
| RC-V (689) | 4.2 | 1.3 | 25.0 | 35.7 | ||
| RC-IV (810) | 8.3 | 1.0 | 10.0 | 11.0 | ||
a
MB, Methanobacteriaceae; MS, Methanosarcinaceae; RC, rice cluster.
b
The same replicate as for construction of the clone library was used.