FIG. 2.

Anaerobic growth of Pseudomonas aeruginosa PAO1 in LB medium supplemented with nitrate. All the inocula were prepared by growing the strains overnight in shaker-grown starter cultures in LB medium, which were then transferred to LB medium supplemented with 1% nitrate and the appropriate concentrations of gentamicin and/or carbenicillin and switched to anaerobic conditions using oxyrase and argon gas. (A) Anaerobic growth of PAO1 (⋄), ΔnarK1::Gm strain (▪), and ΔnarK1::Gm/pnarK1 complemented strain (narK1 complement) (▴). (B) Anaerobic growth of PAO1 (⋄), ΔnarK2::Gm strain (▪), and ΔnarK2::Gm/pnarK2 complemented strain (narK2 complement) (▴). (C) Anaerobic growth of PAO1 (⋄), ΔnarK1K2::Gm strain (▪), and ΔnarK1K2::Gm/pnarK1K2 complemented strain (narK1K2 complement) (▴).