TABLE 3.
Reduced methyl viologen-linked nitrate reductase activities of the P. aeruginosa wild type and mutants grown anaerobically
| Straina | Nitrate reductase activity (nmol nitrite min−1 mg−1 protein)b |
|---|---|
| PAO1 | 334 ± 9 |
| ΔnarK1::Gm strain | 310 ± 10 |
| ΔnarK1::Gm/pnarK1 strain | 330 ± 12 |
| ΔnarK2::Gm strain | 278 ± 12 |
| ΔnarK2::Gm/pnarK2 strain | 328 ± 13 |
| ΔnarK1K2::Gm strain | 229 ± 8 |
| ΔnarK1K2::Gm/pnarK1K2 strain | 283 ± 4 |
a
All strains were grown aerobically in LB medium supplemented with 1% nitrate to an OD660 of 0.5 to 0.6 and then shifted to anaerobiosis for 3 h.
b
Enzyme activities were determined in cell extracts using reduced methyl viologen as the electron donor. Means and standard errors were calculated from three independent cell suspensions.