TABLE 1.
Genetic complementation of E. colia with the ubiE gene of G. stearothermophilus V
| Strain/plasmid | M9 minimal medium supplemented with:
|
|||||
|---|---|---|---|---|---|---|
| glu | succ | glu/cas | succ/cas | glu/ye | succ/ye | |
| AN70/none | +/− | − | + | + | + | + |
| AN70/600prb | + | +/− | + | + | + | + |
a
Cells were streaked in solid M9 minimal medium (9) plates (25 ml) supplemented with the indicated nutrients at 0.2% final concentration and grown at 37°C for 24 h. glu, glucose; succ, succinate; cas, Casamino Acids; ye, yeast extract. −, no growth (even after 48 h); +/−, slow growth; +, normal growth. Trials were done in triplicate.
b
Plates contained ampicillin (100 μg/ml).