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IS900 PCR analysis of DNA extracts from amoebic cultures showing amplification of the predicted 298-bp product, which was also verified by amplicon sequencing. The amoebic cultures had been fed extracts of human gut tissues and then incubated at room temperature for between 35 and 47 months with long periods of encystment. Lane R, reagent control; lane +, positive control; lane L, size ladder.
