. 2006 Jan;72(1):802–810. doi: 10.1128/AEM.72.1.802-810.2006

TABLE 2.

Selected plasmids generated in this study^a

Use and plasmid	Description^b
Complementation and gene introduction
pVSV104	Kn^r, lacZα-(SphI, AvrII, HpaI, SalI, NcoI, KpnI, SacI)
pVSV105	Cm^r, lacZα-(SphI, SalI/HincII, XbaI, SmaI/XmaI, KpnI, SacI)
pVSV106	Tc^r, lacZα-(ApaI, XhoI, SalI/HincII, SmaI/XmaI, XbaI)
pVSV107	Tp^r, lacZα-(KpnI, ApaI, XhoI, SalI, SpeI, XbaI, NotI, SacI)
Copy number variant
pVSV104H	pVSV104 with single-base-pair change in origin and increased copy number
Strain tagging
pVSV102	Kn^r, gfp
pVSV103	Kn^r, lacZ
pVSV208	Cm^r, rfp
Gene expression analyses
pVSV33	Kn^r, transcriptional terminators-(SphI, AvrII, KpnI, XbaI, SalI, StuI)-promoterless Cm^r and gfp
pVSV209	Kn^r, rfp, transcriptional terminators-(AvrII, SalI, StuI)-promoterless Cm^r and gfp
pVSV210	Kn^r, rfp, P_lux-cat-gfp

Other plasmids are described in Materials and Methods.

All plasmids in this table contain the RP4 oriT and the pES213 and R6K_γ replication origins. Kn^r, kanamycin resistance; Cm^r, chloramphenicol resistance; Tc^r, tetracycline resistance; Tp^r, trimethoprim resistance. Parentheses bracket unique restriction sites present in the multiple cloning site.