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RT-PCR to investigate hacA intron splicing. Effect of (A) benzoate treatment on A. niger strains T18-5, T18, and N271 and (B) DTT treatment on A. niger AB4.1. M and M1 represent the λ/HindIII-φX174/HaeII markers (Promega) and 50-bp ladder (Promega), respectively, with relevant marker bands indicated by arrows. DNA fragment intensity was determined densitometrically using TotalLab 1D software (Nonlinear Dynamics). The figure is representative of duplicate biological replicates.
