TABLE 2.
Results of detection of Chlamydiales bacteria in gill cysts of leafy seadragon (Phycodurus eques) and silver perch (Bidyanus bidyanus) and in gill and skin cysts of barramundi (Lates calcarifer)a
| Source | ICC with monoclonal antibodies to Chlamydiaceae LPS | ICC with monoclonal antibody to C. pneumoniae membrane protein | RNA ISH with Chlamydiales 16S rRNA-specific oligoprobe | Partial 16S rRNA sequence code (GenBank accession no.) |
|---|---|---|---|---|
| Leafy seadragon | ± or − | ND | − | CRG20 (AY013396) |
| Silver perch | − | ND | +++ | CRG18 (AY013394) |
| Barramundi | ||||
| Small gill cysts | ++ | ++ | ++++ | CRG98 (AY013474) |
| Larger gill cysts | + or − | + or − | +++ | CRG98 (AY013474) |
| Skin cysts | ± | − | ± (some dots) | CRG98 (AY013474) |
a
Extent of staining: ND, not done; −, none; ±, very weak; +, weak; ++, moderate; +++, intense; ++++, very intense.