TABLE 2.

In vitro alginate synthesis using subcellular fractions of P. aeruginosa FRD1 as a polymerase source^a

Subcellular fraction	cpm/reaction mixture		Alginate polymerase sp act (μU/mg of protein)	In vitro-synthesized alginate (pg)
	No.	%
Crude extract	161	3.8	0.7	0.36
Cytoplasmic membrane	ND	ND	ND	ND
Outer membrane	ND	ND	ND	ND
Envelope	3,037	100	13.2	6.5
Envelopeb	582	19	2.5	1.25

^aAll experiments were performed in triplicates. Average values are given. The standard deviation was <30%. ND, not detectable.

^bTriton X-100 was omitted.