TABLE 1.
Genetic footprinting of E. coli genes related to metabolism of adenylate cofactors NAD(P), CoA, and FAD
| Biosynthetic subsystem | Gene | ORF size (bp) | No. of inserts in ORF in strain:
|
Conclusionb | |
|---|---|---|---|---|---|
| MG1655 | DH10B | ||||
| NAD | nadB | 1,620 | 9 | ND | N |
| nadA | 1,041 | 12 | ND | N | |
| nadC | 891 | 1 | 4 | N | |
| ushA | 1,650 | 6 | ND | N | |
| pnuC | 717 | 4 | ND | N | |
| nadR | 1,251 | 6 | 4 | N | |
| pncA | 657 | 2 | ND | N | |
| pncB | 1,200 | 8 | ND | N | |
| nadD (ybeN) | 639 | 1 | 4 | Ec | |
| nadE | 825 | 0 | 1 | E | |
| nadF (yfiB) | 876 | 0 | 0 | E | |
| CoA | panD | 378 | 2 | 0 | N |
| panB | 792 | 1 | 3 | N | |
| panE | 909 | 5 | 2 | N | |
| panC | 849 | 1 | 5 | N | |
| coaA | 948 | 0 | 0 | E | |
| coaBC (dfp) | 1,290 | 0 | 0 | E | |
| coaD (kdtB) | 477 | 0 | 0 | E | |
| coaE (yacE) | 618 | 0 | 0 | E | |
| acpS | 378 | 0 | 0 | E | |
| FMN/FAD | ribA | 588 | 0 | 0 | E |
| ribD | 1,101 | 0 | 0 | E | |
| ribB | 651 | 0 | 0 | E | |
| ribH | 468 | 0 | 0 | E | |
| ribE | 639 | 0 | 0 | E | |
| ribF (yaaC) | 939 | 0 | 0 | E | |
a
The number of transposon insertions per gene in strains MG1655 and DH10B detected after logarithmic aerobic outgrowth for 23 population doublings in rich medium is shown.
b
Assessments of gene essentiality, based on results of genetic footprinting in strains MG1655 and DH10B. E, essential; N, nonessential; ND, not determined.
c
nadD appeared to be nonessential in the two genetic footprinting experiments but was then shown to be essential by a directed-knockout technique.