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. 2006 Jan;72(1):826–835. doi: 10.1128/AEM.72.1.826-835.2006

FIG. 3.

FIG. 3.

Distribution of Wolbachia in Drosophila embryos. (A and B) Whole-mount immunostainings with rabbit anti-WSP antibody (green) on early-stage embryos of D. simulans infected with wAu (A) and D. willistoni JS 6.3 infected with wWil (B). (C and D) Stage 9 to 10 embryos of D. simulans (C) and D. willistoni (D) are shown in detail. Whereas wAu in D. simulans uniformly infects both somatic and germ line cells, wWil selectively targets a very limited number of primordial germ cells. In D. willistoni, wWil is not detectable at the immunological level in somatic cells at embryonic stage 9 or later. (E to H) Lateral views of a stage 12 embryo of D. willistoni infected with wWil (E and G) and their enhanced magnifications show a row of five heavily infected primordial germ cell nuclei on both lateral sides of the embryo (F and H).