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A, scintillation proximity assay of purified p75NTR with mouse 2.5 S NGF. Best fit is Kd = 2.101 ·10−9 m, with an R2 = 0.9885. B, S200 size exclusion chromatography of p75NTR, NGF, and the p75NTR·NGF complex. The colored bars mark fractions analyzed on SDS-PAGE. Molecular mass calibration is indicated at the top.
