Table 2.

Calpain inhibitors, ALLN and MDL 28170, prevented pre-aggregated Aβ-induced calpain activation in cultured hippocampal neurons

	Treatment
Protease	Aβ	ALLN plus Aβ	MDL plus Aβ
Calpain	236 ± 27*	113 ± 17	106 ± 19

In vitro calpain activity assays were performed using cell extracts obtained from 21-d-in-culture hippocampal neurons treated with calpain inhibitors (ALLN or MDL 28,170) 1 h before the incubation with pre-aggregated Aβ (20 μm) for 8 h. Values are expressed as a percentage of untreated controls, considering the values obtained in these neurons as 100%. The numbers represent the means ± SEM obtained from three experiments. ^*p < 0.01, different from control.