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. Author manuscript; available in PMC: 2006 Jan 27.
Published in final edited form as: J Biol Chem. 2004 Dec 20;280(10):9106–9118. doi: 10.1074/jbc.M411770200

Figure 3. Quantitative slot blot analysis of a subset of genes regulated by inositol and choline.

Figure 3

mRNA from wild type cells grown in the absence of inositol and choline (I−C−), absence of inositol and presence of choline (I−C+), presence of inositol and absence of choline (I+C−), and presence of inositol and choline (I+C+) were analyzed in triplicate by slot blot hybridization as described in the “Experimental Procedures” using the indicated probes. Fold expression for each transcript are normalized to the total counts per minute for ACT1 and expressed as the fraction of the amount of mRNA from cells grown in the absence of inositol and choline. The standard deviation for individual transcripts at each growth condition is indicated. Fold expression of each transcript is plotted in panels A and B using different scales.